High specficity More sensitivity Less background No denaturing step Hot-start Up to 5 kb

DESCRIPTION
BioThermAB HotStart Taq DNA polymerase offers all the benefits of BioTherm DNA polymerase plus an antibody-based, built-in hot start. The BioThermAB polymerase mix contains HotStart Taq Monoclonal Antibody, which blocks polymerase activity prior to the onset of thermal cycling. This prevents primer-dimers and other artifacts resulting from low-level synthesis from nonspecifically primed sites. The antibodies are quickly inactivated by the increased temperature of thermal cycling. BioThermAB polymerase requires no prolonged heating or denaturing step as other hot-start methods do, making BioThermAB polymerase more convenient and easy to use.

APPLICATION
Hot-Start-PCR
PCR applications ranging from cloning to genotyping to mutagenesis.

CONCENTRATION
5 units/µl

UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble formcin 30 min at 72°C.

STORAGE TEMPERATURE
-20°C

10X REACTION BUFER
160 mM (NH₄)₂SO₄, 670 mM Tris-HCl pH 8.8 (at 25°C), 15 mM MgCl₂, 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl₂) is delivered free of charge.

QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases

STORAGE BUFFER
10mM K-phosphate buffer pH 7.0, 100mM NaCl, 0.5mM EDTA, 1mM DTT, 0.01% Tween 20; 50% glycerol (v/v)

PRODUCT CITATIONS
Bakermans-Kranenburg, M. J. & van Ijzendoorn, M. H. (2008), Soc Cogn Affect Neurosci 3(2), 128--134.
Habalová, V.; Klimcáková, L.; Zidzik, J. & Tkác, I. (2009), Mol Cell Probes 23(1), 52--54.
Radvansky, J.; Resko, P.; Surovy, M.; Minarik, G.; Ficek, A. & Kadasi, L. (2010), Anal Biochem 398(1), 126--128.