Robust and reliable Wide range of templates Up to 5 kb

DESCRIPTION
BioTherm™ Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'-3' synthesis of DNA, has no detectable 3'-5' exonuclease (proofreading) activity and possesses low 5'-3' exonuclease activity. In addition, BioTherm™ Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. This property allows easy and efficient ligation of PCR products in TA cloning vectors.

APPLICATION
The enzyme may be used for PCR and for other research and diagnostic applications. BioTherm™ Taq DNA Polymerase can be used for everyday routine applications, even when in low-abundance template conditions (less than 100 DNA molecules of template).

CONCENTRATION
5 units/µl

UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form in 30 minutes at 72ºC under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic acid, sodium salt) pH 9.3 (at 25ºC); 50 mM KCl; 2 mM MgCl₂; 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.

STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)

STORAGE TEMPERATURE
Store BioTherm™ DNA polymerase below 0ºC, preferably at -20ºC, in a constant temperature freezer.

10X REACTION BUFER
160 mM (NH₄)₂SO₄, 670 mM Tris-HCl pH 8.8 (at 25ºC), 15 mM MgCl₂, 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl₂) is delivered free of charge.

1.5 ml 10x reaction buffer (contains 15 mM MgCl₂) Cat. No. GC-002-006
1.5 ml 10x reaction buffer without MgCl₂ plus 50 mM MgCl₂ separately Cat. No. GC-002-007

ASSOCIATED ACTIVITIES
Endonuclease- and exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of 1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72ºC in the presence of 15 - 20 units of BioTherm™ DNA polymerase.

PRODUCT CITATIONS
Castro-Giner, F.; Bustamante, M.; González, J. R.; Kogevinas, M.; Jarvis, D.; Heinrich, J.; Antó, J.-M.; Wjst, M.; Estivill, X. & de Cid, R. (2009), BMC Med Genet 10, 128.
Groeneveld, L. F.; Weisrock, D. W.; Rasoloarison, R. M.; Yoder, A. D. & Kappeler, P. M. (2009), BMC Evol Biol 9, 30.
Wagner, A. O.; Malin, C. & Illmer, P. (2009), Appl Environ Microbiol 75(4), 956--964.